Skip to content Skip to sidebar Skip to footer

Publications

FISH SURVEY DATABASE
environmental DNA, metabarcoding, scDNA

Charron, M.R., Yates, M.C., Heath, D. 2025. Stomach Content DNA (scDNA) Detection and Quantification for Predator Diet Assessment Using High-Throughput Nanofluidic Chip Technology: Species-Specific qPCR Assay Panel Development and Validation. Molecular Ecology Resources :e14106. https://doi.org/10.1111/1755-0998.14106

Sandhu, R., Yates, M., Marques, P., Heath, D., Mandrak, N.E. 2025. Using Environmental DNA Metabarcoding to Determine Endangered Redside Dace Occurrence and Associated Community Composition in Urban Streams. Canadian Journal of Fisheries and Aquatic Sciences 0:0. In Press.

Yates, M.C., Wilcox, T.M., Kay, S., Peres-Neto, P., Heath, D.D. 2025. A Framework to Unify the Relationship Between Numerical Abundance, Biomass, and Environmental DNA. Environmental DNA 7:e70073. https://doi.org/10.1002/edn3.70073

Morey, K., Myler, E., Hanner, R., Tetreault, G. 2024. Taxonomic Blind Spots: A Limitation of Environmental DNA Metabarcoding-Based Detection for Canadian Freshwater Fishes. Environmental DNA 6:e70054. https://doi.org/10.1002/edn3.70054

Gallage, K.S., Van Nynatten, A., Lujan, N.K., Lovejoy, N.R., Mandrak, N.E. 2023. Identifying early life stages of Great Lakes fishes using a metabarcoding approach. Canadian Journal of Fisheries and Aquatic Sciences 80:1813–1823. https://doi.org/10.1139/cjfas-2023-0061

Van Nynatten, A., Gallage, K.S., Lujan, N.K., Mandrak, N.E., Lovejoy, N.R. 2023. Ichthyoplankton metabarcoding: An efficient tool for early detection of invasive species establishment. Molecular Ecology Resources 23:1319–1333. https://doi.org/10.1111/1755-0998.13803

Bernos, T.A., Yates, M.C., Docker, M.F., Fitzgerald, A., Hanner, R., Heath, D., Imrit, A., Livernois, J., Myler, E., Patel, K., Sharma, S., Young, R., Mandrak, N.E. 2023. Environmental DNA (eDNA) applications in freshwater fisheries management and conservation in Canada: overview of current challenges and opportunities. Canadian Journal of Fisheries and Aquatic Sciences 80:1170–1186. https://doi.org/10.1139/cjfas-2022-0162

Mauvisseau, Q., Harper, L.R., Sander, M., Hanner, R.H., Kleyer, H., Deiner, K. 2022. The Multiple States of Environmental DNA and What Is Known about Their Persistence in Aquatic Environments. Environmental Science and Technology 56:5322–5333. https://pubs.acs.org/doi/10.1021/acs.est.1c07638

Nolan, K.P., Loeza‐Quintana, T., Little, H.A., McLeod, J., Ranger, B., Borque, D.A., Hanner, R.H. 2022. Detection of brook trout in spatiotemporally separate locations using validated eDNA technology. Journal of Environmental Studies and Sciences :66–82. https://doi.org/10.1007/s13412-022-00800-x

Wu, Y., Colborne, S.F., Charron, M.R., Heath, D.D. 2022. Development and validation of targeted environmental DNA (eDNA) metabarcoding for early detection of 69 invasive fishes and aquatic invertebrates. Environmental DNA 5:73–84. https://doi.org/10.1002/edn3.359

FISH HEALTH PANEL
transcriptomics, gene expression, stressors

Thraya, M., Patel, A., Stewart, K., Abou-Akl, H., Roberts, D., Heath, D., Pitcher, T.E., Carmona-Alcocer, V., Karpowicz, P. 2025. Integration of photoperiod and time-restricted feeding on the circadian gene expression rhythms in juvenile salmon. Scientific Reports 15:16156. https://doi.org/10.1038/s41598-025-01069-0

Durhack, T.C., Thorstensen, M.J., Mackey, T.E., Aminot, M., Lawrence, M.J., Audet, C., Enders, E.C., Jeffries, K.M. 2025. Behavioural responses to acute warming precede critical shifts in the cellular and physiological thermal stress responses in a salmonid fish (brook trout, Salvelinus fontinalis). Journal of Experimental Biology 228:JEB249964. https://doi.org/10.1242/jeb.249964

Roberts, D., Madliger, C.L., Mokdad, A.I., Firth, B.L., Pitcher, T.E. 2025. Evaluating the effects of probiotic supplementation and structural enrichment prior to transportation on the stress physiology of reintroduced juvenile Atlantic salmon. Environmental Biology of Fishes :. https://doi.org/10.1007/s10641-025-01691-x

Banousse, G., Semeniuk, C., Garant, D., Jeffrey, J., Islam, S.K.S., Bernier, N., Jeffries, K., Audet, C. 2025. Genetic and environmental basis of transcriptional thermal plasticity of brook charr (Salvelinus fontinalis) fry. Canadian Journal of Fisheries and Aquatic Sciences 82:1–15. https://doi.org/10.1139/cjfas-2024-0205

Culbert, B.M., Mossington, E., McCormick, S.D., Bernier, N.J. 2025. Regulation and function of the gill corticotropin-releasing factor system during osmoregulatory disturbances in Atlantic salmon. Journal of Experimental Biology 228:JEB248168. https://doi.org/10.1242/jeb.248168

Culbert, B.M., McCormick, S.D., Bernier, N.J. 2025. Effects of environmental salinity on global and endocrine-specific transcriptomic profiles in the caudal neurosecretory system of salmonid fishes. The FASEB Journal 39:e70477. https://doi.org/10.1096/fj.202403241R

Best, C., Durhack, T.C., Chapelsky, A.J., Aminot, M., Islam, S.S., Heath, D.D., Mochnacz, N.J., Jeffries, K.M. 2025. Transcriptional profiling provides insights on sublethal thermal stress thresholds in juvenile bull trout. Canadian Journal of Fisheries and Aquatic Sciences 0:0. https://doi.org/10.1139/cjfas-2024-0378

Beach, R.K., Dycha, G.M., Wilder, A., Semeniuk, C.A.D., Heath, D.D., Higgs, D.M. 2025. No trout about it: behavioural and transcriptional effects of long-term noise exposure in brook trout (Salvelinus fontinalis). Canadian Journal of Fisheries and Aquatic Sciences 82:1–16. https://doi.org/10.1139/cjfas-2024-0288

Beach, R.K., LeBlanc, J., Higgs, D.M. 2025. An Integrated Approach to Understanding Noise Stress in Two Auditorily Diverse Species of Freshwater Fish. Canadian Journal of Fisheries and Aquatic Sciences 0:0. In Press.

Audet, C., Garant, D., Crespel, A., Vagner, M. 2025. How genomic and environmental relationships shape phenotypic plasticity in brook charr Salvelinus fontinalis: an historical review. Frontiers in Fish Science 3:1525181. https://doi.org/10.3389/frish.2025.1525181

Weinrauch, A.M., Lazaro-Côté, A., Durhack, T.C., Enders, E.C., Jeffries, K.M. 2025. Cellular responses to thermal stress and moderate oxygen limitation in juvenile lake trout. Journal of Fish Biology :1–17. https://doi.org/10.1111/jfb.70111

Wilder, A.S., Wilson, C.C., Warriner, T.R., Semeniuk, C.A.D. 2024. Effects of generations in captivity and elevated rearing temperature on Ontario hatchery brook trout (Salvelinus fontinalis) fry quality and survival. Environmental Biology of Fishes 107:275–292. https://doi.org/10.1007/s10641-024-01528-z

Soto-Dávila, M., Webb, R.A., Rodríguez-Ramos, T., McDonald, G., Dang, X., Heath, J.W., Reid, G., Dixon, B. 2024. Effect of dietary supplementation of probiotic on growth, survival, and immune-related biomarkers in Chinook Salmon (Oncorhynchus tshawytscha) challenged with Vibrio anguillarum. Aquaculture 583:740582. https://doi.org/10.1016/j.aquaculture.2024.740582

Auclert, L.Z., Chhanda, M.S., Derome, N. 2024. Interwoven processes in fish development: microbial community succession and immune maturation. PeerJ 12:e17051. https://doi.org/10.7717/peerj.17051

Amill, F., Gauthier, J., Rautio, M., Derome, N. 2024. Characterization of gill bacterial microbiota in wild Arctic char (Salvelinus alpinus) across lakes, rivers, and bays in the Canadian Arctic ecosystems. Microbiol Spectr 12:e02943-23. https://doi.org/10.1128/spectrum.02943-23

Banousse, G., Normandeau, E., Semeniuk, C., Bernatchez, L., Audet, C. 2024. Parental thermal environment controls the offspring phenotype in Brook charr (Salvelinus fontinalis): insights from a transcriptomic study. G3 Genes|Genomes|Genetics 14:jkae051. https://doi.org/10.1093/g3journal/jkae051

Chhanda, M.S., François, N.R.L., Auclert, L.Z., Derome, N. 2024. Cultivation of Brook Charr Salvelinus fontinalis: The Challenges of Disease Control and the Promise of Microbial Ecology Management. Aquaculture Research 2024:. https://doi.org/10.1155/2024/2279222

Sadeghi, J., Zaib, F., Heath, D.D. 2024. Genetic architecture and correlations between the gut microbiome and gut gene transcription in Chinook salmon (Oncorhynchus tshawytscha). Heredity 133:54–66. https://doi.org/10.1038/s41437-024-00692-3

Frank, C.E., Sadeghi, .J, Heath, D.D., Semeniuk, C.A.D. 2024. Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha). Genes Brain Behav 23:e12898. https://doi.org/10.1111/gbb.12898

Roberts, D., Madliger, C.L., Mokdad, A.I., Pitcher, T.E. 2024. Comparing the stress physiology of hard- and soft-released juvenile Atlantic Salmon after transportation for reintroduction. North American Journal of Fisheries Management 44:1268–1279. https://doi.org/10.1002/nafm.11045

Martínez-Silva, M.A., Dupont-Prinet, A., Houle, C., Vagner, M., Garant, D., Bernatchez, L., Audet, C. 2023. Growth regulation in brook charr Salvelinus fontinalis. General and Comparative Endocrinology 331:114160. https://doi.org/10.1016/j.ygcen.2022.114160

Penny, F., Bugg, W.S., Kieffer, J.D., Jeffries, K.M., Pavey, S.A. 2023. Atlantic Sturgeon and Shortnose Sturgeon exhibit highly divergent transcriptomic responses to acute heat stress. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 45:101058. https://doi.org/10.1016/j.cbd.2023.101058

Jeffrey, J.D., Thorstensen, M.J., Enders, E.C., Treberg, J.R., Jeffries, K.M. 2023. Using transcriptomics to examine the physiological status of wild-caught walleye (Sander vitreus). FACETS 8:1–15. https://doi.org/10.1139/facets-2022-0177

Jourdain-Bonneau, C., Deslauriers, D., Gourtay, C., Jeffries, K.M., Audet, C. 2023. Metabolic and transcriptomic response of two juvenile anadromous brook charr (Salvelinus fontinalis) genetic lines towards a chronic thermal stress. Canadian Journal of Zoology e-First:1–16. https://doi.org/10.1139/cjz-2023-0049

Bendig, T.A., Dycha, G.M., Bull, E.M., Ayala-Osorio, R., Higgs, D.M. 2023. A comparative analysis of form and function in Centrarchidae hearing ability: Does otolith variation affect auditory responsiveness?. The Journal of the Acoustical Society of America 154:772–780. https://doi.org/10.1121/10.0020587

Haniford, L.S.E., LaRochelle, L., Robichaud, J.A., Burton, D., Cooke, S.J. 2023. Evaluating blood, gill, and muscle biopsy methods on the behaviour, reproductive success, and survival of a wild freshwater fish. Applied Animal Behaviour Science 265:106004. https://doi.org/10.1016/j.applanim.2023.106004

Penny, F.M., , Pavey, S.A. 2023. Transcriptomic analyses of juvenile Striped Bass (Morone saxatilis) exposed to chronic and acute temperature change. PLOS ONE 18:e0289372. https://doi.org/10.1371/journal.pone.0289372

Campbell, J.H., Dang, X., Rodríguez-Famos, T., Carpio, Y., Estrada, M.P., Dixon, B. 2023. The effect of PACAP administration on LPS-induced cytokine expression in the Atlantic salmon SHK-1 cell line.. Fish and Shellfish Immunology Reports 5:100116. https://doi.org/10.1016/j.fsirep.2023.100116

Thorstensen, M.J., Vandervelde, C.A., Bugg, W.S., Michaleski, S., Vo, Linh, Mackey, T.E., Lawrence, M.J., Jeffries, K.M. 2022. Non-lethal sampling supports integrative movement research in freshwater fish. Frontiers in Genetics 13:795355. https://doi.org/10.3389/fgene.2022.795355

Semeniuk, C.A.D., Jeffries, K.M., Li, T., Bettles, C.M., Cooke, S.J., Dufour, B.A., Halfyard, E.A., Heath, J.W., Keeshig, K., Mandrak, N.E., Muir, A.J., Postma, L., Heath, D.D. 2022. Innovating Transcriptomics for Practitioners in Freshwater Fish Management and Conservation: Best Practices Across Diverse Resource-sector Users. Reviews in Fisheries and Fish Biology 32:921–939. https://doi.org/10.1007/s11160-022-09715-w

Jeffries, K.M., Jeffrey, J.D., Holland, E.B. 2022. Chapter 8 – Applied aspects of gene function for the conservation of fishes. Fish Physiology 39:389–433. https://doi.org/10.1016/bs.fp.2022.04.008

Jeffries, K.M., Michaleski, S., Bernier, N.J., Heath, D.D., Miller, K.M., Teffer, A. 2021. The use of non-lethal sampling for transcriptomics to assess the physiological status of wild fishes. Comparative Biochemistry and Physiology, Part B 256:110629. https://doi.org/10.1016/j.cbpb.2021.110629

Turko, A.J., LeClair, A.T.A., Mandrak, N.E., Drake, D.A.R., Scott, G.R., Pitcher, T.E. 2021. Choosing source populations for conservation reintroductions: lessons from variation in thermal tolerance among populations of the imperilled redside dace. Canadian Journal of Fisheries and Aquatic Sciences 78:1347–1355. http://dx.doi.org/10.1139/cjfas-2020-0377

Turko, A.J., Nolan, C.B., Balshine, S., Scott, G.R., Pitcher, T.E. 2020. Thermal tolerance depends on season, age and body condition in imperilled redside dace Clinostomus elongatus. Conservation Physiology 8:coaa062. https://doi.org/10.1093/conphys/coaa062

DECISION-GUIDING TOOLS
challenges, media, ethics

A. Howarth, Cooke, S.J., V.M. Nguyen 2025. It takes all kinds: a composite approach to sustainability in Canadian freshwater fisheries. Canadian Journal of Fisheries and Aquatic Sciences 0:0. In Press.

Howarth, A., Nguyen, V.M., Cooke, S.J. 2023. Managing Canadian freshwater fisheries: persistent challenges and emerging opportunities. Canadian Journal of Fisheries and Aquatic Sciences 80:1436–1455. http://dx.doi.org/10.1139/cjfas-2023-0011

Piczak, M.L., Brooks, J.L., Bard, B., Bihun, C.J., Howarth. A., Jeanson, A.L., LaRochelle, L., Bennett, J.R., Lapointe, N.W.R., Mandrak, N.E., Cooke, S.J. 2022. Revisiting the challenge: perspectives on Canada’s freshwater fisheries policies three decades after the Pearse Report. FACETS 7:912–935. https://doi.org/10.1139/facets-2021-0145

Fitzgerald, A., Heath, D., Halliday, J. 2021. Environmental DNA as Novel Technology: Lessons in Agenda Setting and Framing in News Media. Animals 11:2874. https://doi.org/10.3390/ani11102874