{"id":1751,"date":"2022-06-30T12:41:24","date_gmt":"2022-06-30T16:41:24","guid":{"rendered":"https:\/\/gen-fish.ca\/?page_id=1751"},"modified":"2025-07-07T16:12:11","modified_gmt":"2025-07-07T20:12:11","slug":"equipment-and-protocols","status":"publish","type":"page","link":"https:\/\/gen-fish.ca\/fr\/equipment-and-protocols\/","title":{"rendered":"Equipment and Protocols"},"content":{"rendered":"
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COLLECTING AND PROCESSING SAMPLES<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Equipment and Protocols<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Consistency among sample collection and processing can be critical to the efficiency of a genomics project and the long-term reliability of the results. Below are the recommended considerations and protocols developed by GEN-FISH.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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FIELD WORK<\/h3><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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LAB WORK<\/h3><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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These equipment protocols and checklists should be reviewed before eDNA sampling. A completed sanitization and packing checklist is recommended for each unit after use.<\/p>

OSMOS Pre-Prime Instructions<\/strong><\/a><\/span><\/p>

OSMOS Protocol and Checklist<\/strong><\/span><\/a><\/p>

DIY Peristaltic Pump Protocol and Checklist<\/strong><\/a><\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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Our YouTube channel offers how-to videos for handling and operating eDNA sampling equipment.<\/p>

Unpacking & Packing | OSMOS<\/strong><\/span><\/a><\/p>

eDNA Sampling Protocol | OSMOS<\/strong><\/span><\/a><\/p>

eDNA Sampling Protocol | DIY Peristaltic Pump<\/strong><\/span><\/a><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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There are a number of important considerations for eDNA sample collection:<\/p>

GEN-FISH eDNA Sampling Guidance and Considerations<\/strong><\/a><\/span><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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Sample metadata and environmental parameters are also important for interpreting the collected eDNA data. The instructions let you know (through a colour code) which data is “crucial”, “important”, and “nice to have”.<\/p>

GEN-FISH Field Sheet Instructions<\/strong><\/a><\/span><\/p>

Lentic Field Sheets<\/strong><\/a><\/span><\/p>

Lotic Field Sheets<\/strong><\/a><\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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These are instructions for designing OpenArray chips, based on Thermo Fisher protocols and using Primer Express software.<\/p>

OpenArray Assay Development<\/strong><\/a><\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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RNAlater has been extensively used to store animal tissues, including brain, heart, kidney, spleen, liver, testis, muscle, fat, lung, and thymus. It penetrates into most biological tissues, and immediately stabilizes RNA (and DNA) in fresh specimens.<\/p>

GEN-FISH RNAlater Protocol<\/strong><\/a><\/span><\/p><\/div><\/div><\/div><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t

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The protocols developed by the Docker Lab (University of Manitoba) are excellent standards for extracting eDNA from filters and DNA from tissues.<\/p>

eDNA Filter Processing Protocol<\/strong><\/a><\/span><\/p>

DNA Extraction Protocol<\/strong><\/a><\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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COLLECTING AND PROCESSING SAMPLES Equipment and Protocols Consistency among sample collection and processing can be critical to the efficiency of a genomics project and the long-term reliability of the results.…<\/p>","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"nf_dc_page":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-1751","page","type-page","status-publish","hentry"],"yoast_head":"\nEquipment and Protocols » GEN-FISH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/gen-fish.ca\/fr\/equipment-and-protocols\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta 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