{"id":2921,"date":"2025-07-07T23:04:15","date_gmt":"2025-07-08T03:04:15","guid":{"rendered":"https:\/\/gen-fish.ca\/?p=2921"},"modified":"2025-07-07T23:04:18","modified_gmt":"2025-07-08T03:04:18","slug":"development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank","status":"publish","type":"post","link":"https:\/\/gen-fish.ca\/fr\/development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank\/","title":{"rendered":"Development of Taxonomic and Regional eDNA Assays for qPCR &#8211; Chelsea Frank"},"content":{"rendered":"<p class=\"\"><strong>Author:<\/strong> Chelsea E. Frank<\/p>\n\n\n\n<p class=\"has-text-align-justify\">Environmental DNA (eDNA) is a useful tool for management of aquatic ecosystems, as its applications provide a non-invasive method for species detection. Compared to other molecular technologies (e.g., metabarcoding) for analyzing target species eDNA presence\/absence, OpenArray<sup>TM<\/sup> chips provide a time and cost-effective quantification method. As part of Activity 1, our goal at GEN-FISH was to determine the location and abundance of over 200 of Canada\u2019s freshwater fish species with our fish survey toolkit. As part of this toolkit, we implemented a species-specific approach to designing and optimizing OpenArray<sup>TM<\/sup> qPCR assays for taxonomically (e.g., salmonid and non-teleost) and\/or geographically (e.g., Atlantic Canada, Western Canada, the Prairies, and Southern Ontario) related freshwater fishes. Initially we developed OpenArray<sup>TM<\/sup> chips to test the sensitivity and specificity of COI and CytB primer sets at a rapid pace. This testing was then followed by utilizing the remaining OpenArray<sup>TM<\/sup> chips to determine species presence via eDNA quantification, from over 500 water bodies across Canada. While we are limited in the focus of species in which primer sets appropriate for OpenArray<sup>TM<\/sup> technologies have been developed, this enables us to take a more direct approach in aquatic management with respect to breadth of application (e.g., conservation and management). With a specific number of species-specific assays within a chip, we can highlight the focus of these technologies to provide information on species of concern (e.g., invasive or endangered) without harm to the species present within those environments.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"aligncenter wp-block-button\"><a class=\"wp-block-button__link has-white-color has-vivid-red-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/youtu.be\/1iYnpeo89NU?feature=shared&amp;t=6524\" style=\"border-radius:100px\">WATCH THIS PRESENTATION<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"\"><\/p>","protected":false},"excerpt":{"rendered":"<p>Author: Chelsea E. Frank Environmental DNA (eDNA) is a useful tool for management of aquatic ecosystems, as its applications provide a non-invasive method for species detection. Compared to other molecular&hellip;<\/p>","protected":false},"author":8,"featured_media":2923,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[74,57],"tags":[],"class_list":["post-2921","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2025_agm","category-abstracts"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Development of Taxonomic and Regional eDNA Assays for qPCR - Chelsea Frank &#187; GEN-FISH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/gen-fish.ca\/fr\/development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank\/\" \/>\n<meta property=\"og:locale\" content=\"fr_CA\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Development of Taxonomic and Regional eDNA Assays for qPCR - Chelsea Frank &#187; GEN-FISH\" \/>\n<meta property=\"og:description\" content=\"Author: Chelsea E. Frank Environmental DNA (eDNA) is a useful tool for management of aquatic ecosystems, as its applications provide a non-invasive method for species detection. Compared to other molecular&hellip;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/gen-fish.ca\/fr\/development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank\/\" \/>\n<meta property=\"og:site_name\" content=\"GEN-FISH\" \/>\n<meta property=\"article:published_time\" content=\"2025-07-08T03:04:15+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-07-08T03:04:18+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/gen-fish.ca\/wp-content\/uploads\/2025\/07\/Frank-C_AGM-2025_Presentaion.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1738\" \/>\n\t<meta property=\"og:image:height\" content=\"978\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Paige W. Breault\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u00c9crit par\" \/>\n\t<meta name=\"twitter:data1\" content=\"Paige W. Breault\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimation du temps de lecture\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/gen-fish.ca\\\/development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/gen-fish.ca\\\/development-of-taxonomic-and-regional-edna-assays-for-qpcr-chelsea-frank\\\/\"},\"author\":{\"name\":\"Paige W. 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